Summary
Type: TLC
Application #: 403380
Catalog#: 1122026 - Cellulose 300 PEI TLC Plates, polyester backed, 100um, 20x20cm 25/pk
Detection: Autoradiographic exposure of x-ray film, 16 h
Conditions: Applied volume: 5-20 μl
Washing: For a removal of salts and dehydrates the plates are immersed in methanol for 5 min and then allowed to dry at room temperature.
First Development: Develope plates in first dimension with below described solvents.
Washing: Wash again with methanol for 20 min.
Second Development: After drying develope the plates in second dimension with below described solvents.
Chromatographic solvents:
Ta - 0.75 M Tris, 0.45 M HCl
Tb - 0.75 M Tris, 0.45 M HCl, 0.5 M LiCl
Sa - 74 g of (NH4)2SO4, 0.4 g of (NH4)HSO4, 100 ml of H2O
Sb - 74 g of (NH4)2SO4, 0.4 g of (NH4)HSO4, 4 g of Na2EDTA, 100 ml of H2O
Sc - 3 M (NH4)2SO4
Ga - 0.9 M guanidine HCl
Gb - 0.4 M guanidine HCl
Aa - 1 M acetic acid, pH adjusted to 3.5 with NH4OH
Ma - 1.5 M morpholine, 1.2 M boric acid
Pa - 0.75 M KH2PO4, pH adjusted to 3.5 with 0.75 M H3PO4
Pb - 0.4 M K2HPO4, 0.7 M boric acid
Fa - 4 M formic acid, pH adjusted to 3.5 with NH4OH
Authors: Bochner B R Ames B N
Source: The Journal of Biological Chemistry Vol 257 No 16 pp 9759-9769 1982